Complex of mulberry extract (CME) is composed of extracts of mulberry (Morus alba L.) fruit, mulberryleaves and black beans (Glycine max (L.) Merr.). In this study, we investigated prevention effects of CME on degenerativearthritis. The SC50 value of DPPH radical scavenging by CME was 158.49±11.35㎍/mL. We found that CME significantlyreduced the production of nitric oxide (NO) and protein expression of cyclooxygenase-2 (COX-2) in RAW 264.7 cells whichwere activated by LPS. Experiments using animal model of degenerative arthritis showed that CME (400㎎/㎏ bodyweight) inhibited the production of TNF-α (77.5%) and IL-1β (95.0%). Furthermore, it was observed that CME reduced to85.9% of paw edema induced by carrageenan. These results suggest that CME could improve degenerative arthritis.

 ABSTRACT  서 언  재료 및 방법   1. 상심자추출분말의 제조   2. 상엽추출분말의 제조   3. 서리태추출분말의 제조   4. Complex of Mulberry Extract (CME)의 제조   5. DPPH Radical 소거능 측정   6. 세포주 배양   7. 세포 (Raw 264.7) 독성 측정   8. Lipopolysaccharide (LPS) 유도 NO 생성량 측정   9. Western Blotting에 의한 COX-2의 발현 측정   10. MIA 유도 퇴행성관절염 동물모델 제작 및 CME 경구투여   11. TNF-α 및 IL-1β 생성억제 측정   12. Carrageenan 유도 급성염증 동물모델 제작 및 CME경구투여   13. 통계학적 분석  결과 및 고찰   1. DPPH Radical 소거능 측정   2. 세포 (Raw 264.7) 독성 측정   3. Lipopolysaccharide (LPS) 유도 NO 생성량 측정   4. TNF-α 및 IL-1β 생성억제 측정   5. COX-2의 세포내 발현억제 측정   6. Carrageenan 유도 급성염증 동물모델의 족부 부종억제 측정  REFERENCES

• 서형호(㈜벤스랩 중앙연구소) | Seo, Hyeong Ho
• 정종문( 수원대학교 생명과학과) | 정종문