Pleurotus has increased rapidly production and consumption because of highly nutritional value, natural healthy food and so on. The basic studies for Pleurotus need for development of mushroom industry. This study was to investigate the cultural characteristics among 15 strains of 6 species and to analyze their phylogenetic relationships. The cultural characteristics were investigated by mycelial growth activity at different media, temperature and pH. The optimum media for mycelial growth were YM and MCM in most species. The optimum temperature for mycelial growth were 25˚C and 30˚C. The optimum pH for mycelial growth were widly range from pH 5.1 to 7.4. Through the RFLP (restriction fragment length polymorphism) of IGS (intergenic spacer) I region in ribosomal DNA, it was analyzed phylogeny of interspecies and intraspecies. Each species was discriminated well as isolates within each species formed clade to be distinguished other species. P. florida was highly similar to P. floridanus, and P. flabellatus was P. cornucopiae. P. fuscus var. ferulae was highly similar to P. eryngii but discriminated different species in analysis of RFLP of IGS I region and showed different characteristics in mycelial culture. RFLP of IGS I region was useful of studying phylogenetic relationships of species and population.

 ABSTRACT  Introduction  Material and Methods   Fungal Strains   Media   Temperature   pH   Sample preparation and genomic DNA extraction   PCR amplification of IGS I region in rDNA   Restriction enzyme digestion and electrophoresis   Phylogenetic analysis  Results and Discussion   Cultural Characteristics   Phylogenetic relationships based on RFLP of IGS I region in rDNA  Acknowledgements  References

• Choi, Sun-Gyu(Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
• Jang, Kab-Yeul( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) | Jang, Kab-Yeul
• Kim, Gyu-Hyun( Dep. of Biotechnology, Cheonan Yonam College) | Kim, Gyu-Hyun
• Kong, Won-Sik( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) | Kong, Won-Sik
• Jo, Jae-Sun( Dep. of Food Science and Biotechnology, Kyung Hee University) | Jo, Jae-Sun
• Kim, Hae-Yeong( Dep. of Food Science and Biotechnology, Kyung Hee University) | Kim, Hae-Yeong
• Yoo, Young-Bok( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) | Yoo, Young-Bok