Oyster mushrooms including of P. ostreatus, P. eryngii, P. pulmonarius and P. cornucopiae are one of the famous mushrooms for foods in Korea. RAPD were carried out using 14 of oligoprimers to analyze the phylogenetic relationship among 57 strains of 32 Pleurotus species. Most of species formed the minimum clade with strains within species and was divided respectively species. Therefore clade was separated well in accordance species. Pleurotus species formed again clade to be added in close related to other species, and were discriminated by sixteen clades with each representative species including high similarity groups. Sixteen clades were composed representative species according to each clade. There were clade I of P. pulmonarius(P. sajor-caju, P. opuntiae, P. sapidus), clade II of P. eryngii(P. fuscus var. ferulae, P. fossulatus), clade III of P. ostreatus(P. ostreatus var. columbinus, P. spodoleucus, P. floridanus), clade IV of P. florida, clade V of P. djamor(P. flabellatus, P. incarnates, P. salmoneo-stramineus), clade VII of P. populinus(P. subareolatus), clade VIII of P. cystidiosus(P. cystidiosus var. formosensis), clade X of P. dryinus(P. dryinus var. pometi), clade XIV of P. cornucopiae(P. citrinopilieatus, P. euosmus), and clade XV of P. australis. These species were representative species each clades. Five species, P. ulmarius(clade VI), P. griseus(clade IX), P. calyptratus(clade XI), P. lampas(clade XII), P. smithii(clade XIII)and P. serotinus (clade XVI) were used each one strain in analysis, so they were clustered other groups.

Pleurotus has increased rapidly production and consumption because of highly nutritional value, natural healthy food and so on. The basic studies for Pleurotus need for development of mushroom industry. This study was to investigate the cultural characteristics among 15 strains of 6 species and to analyze their phylogenetic relationships. The cultural characteristics were investigated by mycelial growth activity at different media, temperature and pH. The optimum media for mycelial growth were YM and MCM in most species. The optimum temperature for mycelial growth were 25˚C and 30˚C. The optimum pH for mycelial growth were widly range from pH 5.1 to 7.4. Through the RFLP (restriction fragment length polymorphism) of IGS (intergenic spacer) I region in ribosomal DNA, it was analyzed phylogeny of interspecies and intraspecies. Each species was discriminated well as isolates within each species formed clade to be distinguished other species. P. florida was highly similar to P. floridanus, and P. flabellatus was P. cornucopiae. P. fuscus var. ferulae was highly similar to P. eryngii but discriminated different species in analysis of RFLP of IGS I region and showed different characteristics in mycelial culture. RFLP of IGS I region was useful of studying phylogenetic relationships of species and population.

A total of 35 phosphate solubilizing bacterial strains were isolated from waste mushroom bed of Agar-icus bisporus in Buyeo-Gun, Chungnam and screened for the production of indole acetic acid (IAA). The best IAAproducing strain was identified as Pantoea rodasii using 16S rRNA analysis. In addition to the IAA production, thisstrain could act as an efficient phosphate solubilizer (1100µg ml-1 after 5days of incubation) also. The selected strainwas cultured under different conditions in order to assess the optimum conditions for maximum IAA production. Thenutrient broth (NB) medium was recorded as the best medium, where the maximum IAA production (229µg ml-1)was recorded at the start of stationary phase (12hours after inoculation) of the bacteria growth. The performanceof the strain was found to be maximum at the temperature of 30℃ followed by 25℃. IAA production was foundto be increased with increasing tryptophan concentration (from 0.1 to 0.6%), however beyond this limit, a slight reduc-tion in IAA production was observed. The strains’ ability to produce IAA was further confirmed by extraction ofcrude IAA and subsequent TLC analysis. A specific spot from the extracted IAA preparation was found correspondingwith the standard spot of IAA with same Rf value. The results of HPLC analysis conducted in identifying and quan-tifying the IAA production more precisely, are in agreement with the results of the assessment done with colorimetricmethod. As revealed by the results of the pot experiment, the isolated strain could significantly enhance the growth(as measured by shoot and root growth) of mung bean plants compared to that of non-inoculated plants. Thereforeit can be concluded that the present strain, Pantoea rodasii has great potential to be used as bio-inoculants.