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해충저항성 Bacillus thuringiensis (Bt) 벼 급이가 벼물바구미(Lissorhoptrus oryzophilus)의 살충제 감수성에 미치는 영향 KCI 등재
Effect on Insecticide Susceptibility of Lissorhoptrus oryzophilus Fed on Effect on Insecticide Susceptibility of Lissorhoptrus oryzophilus Fed on
pp.247-253
해충저항성 Bacillus thuringiensis (Bt) 벼의 비표적곤충인 벼물바구미(Lissorhoptrus oryzophilus)에 대한 성충 전용살충제 Clothianidin 액상수화제의 살충제 감수성 시험을 실시한 결과, 72시간-LC50은 0.245 ml/L(95% 신뢰한계는 0.195~0.307 ml/L)이었으며, Bt벼의 모본으로 대조로 사용한 낙동벼의 72시간-LC50은 0.257 ml/L(95% 신뢰한계는 0.199~0.331 ml/L)이었다. 72시간-LC50은 낙동벼에서 다소 높았지만, 해충저항성 Bt벼 72시간-LC50이 낙동벼의 95% 신뢰한계 내에 포함되어, 두 품종의 LC50값에 유의성이 없는 것으로 판단된다.
Insect-resistant transgenic rice, Bt rice was developed by inserting the mCry1Ac1, a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For the biosafety assessment of this GM rice, we studied the effect on insecticide susceptibility of Rice Water Weevil (Lissorhoptrus oryzophilus) which is a nontarget insect pest and commonly used as a model organism in ecotoxicological studies. The Bt rice was confirmed to have the insertion of T-DNA and the protein expression by the polymerase chain reaction (PCR) and the ELISA analysis. As for the cumulative mortalities of Rice Water Weevil on insecticide, Clothianidin in Bt and non-GM counterpart, Nakdong rices were similar. The 72h-LC50 values of Bt and non-GM rices showed 0.245 ml/L and 0.257 ml/L respectively. As the 72h-LC50 vlaue of Bt rice was included within the 95% confidence limit of that of Nakdong rice slightly higher, the two varieties of the 72h-LC50 values were not significant. Thus, we found out there was a strong possibility that Bt rice didn't affect the insecticide susceptibilities of nontarget insects.
