논문 상세보기
pp.145-150
미강으로부터 분리 정제된 피틴산을 식육에 적용하여 식육에서의 항균활성과 항산화능을 조사함으로써 식품보존제로서의 가능성을 확인하였다. 피틴산이 첨가된 Tryptic soy broth에 S. Typhimurium KCCM 11806을 배양하였을 경우 1%(w/v) 피틴산 첨가 시 S. Typhimurium의 생육을 완전히 저해시켰다. E. coli O157:H7에 대한 항균 효과는 1.0%(w/v) 피틴산 첨가 시 약 3.0 log 단위의 생육저해효과를 확인하였다. 신선한 닭고기, 돼지고기, 소고기에 S. Typhimurium을 접종하고 피틴산을 첨가하여 37oC에서 24시간 배양하여 피틴산의 항균효과를 조사한 결과 모든 식육에서 항균효과가 피틴산 농도에 의존적으로 나타났으며 1%(w/v) 피틴산 첨가 시 약 3.0 log 단위의 항균효과를 나타내었다. 생육인 닭고기, 돼지고기, 소고기에 피틴산을 여러 농도로 첨가한 결과 E. coli O157:H7에 대한 항균효과는 모든 생육에서 0.5% 피틴산 첨가 시 약 2.0 log 단위의 생육저해 효과를 보여 주었으며, 가열 처리된 식육에서도 첨가된 피틴산 농도에 의존적으로 유의성 있게 증식을 억제하였다. 생육에 피틴산을 첨가하여 4oC에서 3일간 저장할 경우 저장기간이 증가함에 따라 TBARS(thiobarbituric acid-reactive substances) 값이 증가하였으며 피틴산을 5 mM 첨가하였을 때 TBARS 값이 유의적으로 감소하여 식육에서의 항산화능을 확인하였다.
Phytic acid was purified from rice bran and applied to meats. Then, its antimicrobial and antioxidant activities were examined to investigate its availability as a food preservative. When Salmonella Typhimurium KCCM 11806 was cultivated in tryptic soy broth added with phytic acid, the growth of S. Typhimurium was completely inhibited at the concentration of 1% (w/v) phytic acid. Similarly, the growth of Escherichia coli O157:H7 was reduced by 3.0 log scale when 1% (w/v) phytic acid was added to the growth medium. When Salmonella Typhimurium was inoculated onto fresh chicken, fork, and beef supplemented with phytic acid followed by incubation at 37oC for 24 hr, the antimicrobial activity of phytic acid was dependent on the concentration of phytic acid, and the growth of Salmonella Typhimurium was reduced by 3 log scale at 1% (w/v) phytic acid. When phytic acid was applied to the fresh chicken, fork, and beef, the growth of E. coli O157:H7 was reduced by 2.0 log scale at the concentration of 0.5% (w/v) phytic acid. Phytic acid also inhibited the growth of E. coli O157:H7 inoculated on cooked meats in a concentration-dependent manner. When fresh meats supplemented with phytic acid were stored at 4oC for 3 days, TBARS (thiobarbituric acid-reactive substances) values of fresh meats increased as the storage time increased, and decreased significantly at the concentration of 5 mM phytic acid, indicating that phytic acid has antioxidant activity.
