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Phylogenic Analysis of Endosymbiont Wolbachia with Aedes albopictus (Diptera: Culicidae) in Korea

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  • URLhttps://db.koreascholar.com/Article/Detail/287690
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

The intracellular endosymbiont bacterium Wolbachia is currently considered the most abundant in arthropods and has also been isolated from nematodes, amphipods, isopods, mites and spiders. Recently, Wolbachia-based research was focused on the control of disease vector-population, such as several mosquitoes such as genus Aedes and Anopheles which cause dengue fever and malaria, respectively. For the analysis of regional difference between vector mosquito Aedes albopictus and Wolbachia, we selected different regions and collected Ae. albopictus which were distinguished with mountain chain and waterway. Whole genomic DNA were extracted from collected specimens with 9 regions. PCR analysis and sequencing were accomplished in each specimen for Wolbachia detection and identification using WSP gene. As a results, almost mosquitoes were infected with two strain of Wolbachia both wAlbA and wAlbB. However, regional separation of vector mosquitoes, wAlbA strain of Wolbachia were showed more than 98% sequence similarity. In this study, we first reported that Wolbachia infection and type of Wolbachia in Korea and endosymbiont Wolbachia was showed highly sequence homologies.

저자
  • Chan Hee Park(Division of Medical Entomology, Center for Immunology and Pathology, Korea National Institute of Health)
  • Hyung Woo Lim(Division of Medical Entomology, Center for Immunology and Pathology, Korea National Institute of Health)
  • Hyun Woo Kim(Division of Medical Entomology, Center for Immunology and Pathology, Korea National Institute of Health)
  • Wook Gyo Lee(Division of Medical Entomology, Center for Immunology and Pathology, Korea National Institute of Health)
  • Jong Yul Roh(Division of Medical Entomology, Center for Immunology and Pathology, Korea National Institute of Health)
  • Mi Yeoun Park(Division of Medical Entomology, Center for Immunology and Pathology, Korea National Institute of Health)
  • E-Hyun Shin(Division of Medical Entomology, Center for Immunology and Pathology, Korea National Institute of Health)