Silk fibroin (SF) and silk sericin (SS) have been studied as a biomaterial due their useful properties including good blood compatibility and good cell activities. However, previous studies, silkworm variety has not been considered although it can affect structure and properties of silk fibroin and sericin. Therefore, in the present study, structure and properties of silk fibroins and sericins obtained from different silkworm variety were examined. Most of regenerated SF solutions from different silkworm varieties displayed Newtonian fluid behaviors and their viscosities were different depending on the silkworm variety. N74 showed the highest viscosity among the SF samples. Molecular weight (MW) distribution and mechanical properties of regenerated SF showed similar result to viscosity result. SS did not show significant difference in MW distribution and viscosity result depending on the silkworm variety.