Sacbrood virus (SBV) is one of the most fatal pathogens against Asian honeybee, Apis cerana. This virus cause failure of the insect larvae to pupate and death of the adult insects. This study has analyzed the host genes affected by viral infection, by comparing the expression level of host transcripts infected with or without SBV. As a first step, we sequenced the cDNA libraries of Asian honeybee by using illumina RNA sequencing. The sequences were de novo assembled to acquire honeybee transcriptome sequences. The transcriptome was annotated by the sequence comparison to known protein sequences by BLASTX and evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG) database with functional categories and description. By mapping the RNA-seq data to de novo assembled transcripts, we characterized the differentially expressed transcripts between SBV-infected and non-infected Asian honeybee.

• Seok Hee Lee(Department of Agricultural Biotechnology, Seoul National University)
• Sang Woon Shin(Department of Agricultural Biotechnology, Seoul National University)
• Qin Liu(Department of Agricultural Biotechnology, Seoul National University)
• Song Eun Kim(Department of Agricultural Biotechnology, Seoul National University)
• Saes Byeol An(Department of Agricultural Biotechnology, Seoul National University)
• Fang Ying(Department of Agricultural Biotechnology, Seoul National University)
• Jae Young Choi(Research Institute for Agriculture and Life Sciences, Seoul National University)
• Woo Jin Kim(Research Institute for Agriculture and Life Sciences, Seoul National University)
• Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University)