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Molecular Characterization of the Mosquitocidal cry Genes from a Bacillus thuringiensis Serovar mogi

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  • URLhttps://db.koreascholar.com/Article/Detail/289117
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

Plasmids from Bacillus thuringiensis have been implicated in pathogenicity as they carry the genes responsible for different types of diseases in mammals and insects. B. thuringiensis serovar mogi of a novel serogroup (H3a3b3d), which showed mosquitocidal activity against Anopheles sinensis and Culex pipiens pallens, was isolated from fallen leaves in Mungyeong city, Republic of Korea. In contrast to the complicated plasmid profiles of B. thuringiensis H3 serotype strains, the B. thuringiensis serovar mogi contained only megaplasmid (> 30 MDa) on which the toxin genes were occasionally located. Sequence analysis using 454-pyrosequencing revealed that the megaplasmid harbored at least seven putative cry genes, showing about 84%, 75%, 73%, 58%, 84%, 39% and 75% similarities in amino acid sequences with Cry27Aa, Cry19Ba, Cry20-like, Cry56Aa, Cry39ORF2, Cry8Ba and Cry40ORF2, respectively. These cry genes were cloned to the Escherichia coli-B. thuringiensis shuttle vector, pHT1K, and then introduced into an acrystalliferous B. thuringiensis Cry-B strain for further molecular characterization. This novel 3a3b3d type strain, B. thuringiensis serovar mogi, could be used as a good resource for studying unknown mosquitocidal cry genes.

저자
  • Qin Liu(Department of Agricultural Biotechnology, Seoul National University)
  • Jae Young Choi(Department of Agricultural Biotechnology, Seoul National University)
  • Xue Ying Tao(Department of Agricultural Biotechnology, Seoul National University)
  • Joo Hyun Lee(Department of Agricultural Biotechnology, Seoul National University)
  • Song Eun Kim(Department of Agricultural Biotechnology, Seoul National University)
  • Zhenli Fu(Department of Agricultural Biotechnology, Seoul National University)
  • Jae Su Kim(Department of Agricultural Biology, Chonbuk National University)
  • Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University)