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Discrimination of acaricidal resistance of Tetranychus urticae using surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS)

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  • URLhttps://db.koreascholar.com/Article/Detail/289395
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

This study was conducted to examine the potential of surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to screen Tetranychus urticae resistance to pyridaben and dicofol. T. urticae is one of the most important pests in greenhouse and orchard, and huge expense is needed to control because of its strong resistance to acaricides. Consequently speedy and accurate monitoring of acaricidal resistance is the key factor of IPM for T. urticae. SELDI-TOF MS is a novel approach to biomarker discovery that combines two powerful techniques: chromatography and mass spectrometry. It can provide a rapid protein expression profile of acaricidal sensitive and resistant T. urticae. In this study we had different protein and peptide patterns between sensitive and resistant strains to pyridaben and dicofol. In the future this results could be a useful data to develop a good monitoring tool of site and host specific mite resistance to various acaricides.

저자
  • Hyoung-ho Mo(Department of Agricultural biology, Korea University)
  • Myeongseop Kim(Division of Environmental Science and Ecological Engineering, Korea University)
  • Jung-Joon Park(Institute of Life Science and Natural Resources, Korea University)
  • Kijong Cho(Division of Environmental Science and Ecological Engineering, Korea University)