The cotton aphid, Aphis gossypii (Glover), is one of the most serious pest in seed potato and various vegetable cultivation. The imidacloprid resistant strain (IR) was over 200 fold more resistant to imidacloprid compared to the susceptible strain (S) as judged by the LC50 values and IR showed cross resistant to acetamiprid, thiamethoxam, thiacloprid, clothianidin. By using the suppression subtractive hybridization method, a imidacloprid resistant associated cDNA library was constructed in adult cotton aphid. In total 115 differentially expressed cDNA clones were obtained. Any point mutation detected in nicotinic acetylcholine receptor alpha 1~5 and beta 1 subunits in the IR. Based on IEF, the IR general esterase isozyme banding patterns were identical with that of S.

• Ju Il Kim(Highland Agriculture Research Center, NICS, RDA)
• Min Kwon(Highland Agriculture Research Center, NICS, RDA)
• Jae Soon Kang(School of Agricultural Biotechnology, Seoul National University)
• Si Hyeock Lee(School of Agricultural Biotechnology, Seoul National University)
• Si Woo Lee(Applied Entomology Division, NIAS, RDA)