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Molecular Characterization of A Genomic DNA of Bacillus thuringiensis Bacteriophage and Its Distribution in Bacillus thuringiensis Type Strains

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  • URLhttps://db.koreascholar.com/Article/Detail/290372
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

Through an application of plasmid capture system (PCS) to Bacillus thuringiensis plasmid DNAs, we acquired 21 polymorphic clones of putative genomic DNA of bacteriophage. The genome size of phage 1-3 (PhBT1-3) was determined to be 46,517 base pairs (bp) with 35.43% G + C content and 83% coding region. Sixty-five putative open reading frames (ORFs) with more than 50 codons were found in the new phage genome. In accordance with this genome finding, the phage particles and its DNA were confirmed from the supernatant of B. thuringiensis 1-3. Morphological characterization and infectivity assay demonstrated that PhBT1-3 belongs to the family Siphoviridae and it showed infectivity to three B. thuringiensis type strains, galleriae, entomocidus, and morrisoni. Based on these results, we screened the existence of phages in B. thuringiensis type strains by PCR with terminase small subunit-specific primers. Ten of 67 type strains showed PCR products and the similarity of those putative amino acids was more than 70%. Furthermore, we verified the existence of various shaped phages from the supernatants of 10 B. thuringiensis type cultures. In conclusion, we characterized a putative genome of phage, PhBT1-3 from B. thuringiensis 1-3, and confirmed the distribution of phages in the group of 67 B. thuringiensis type strains.

저자
  • Jong Yul Roh(Department of Agricultural Biotechnology, Seoul National University)
  • Qin Liu(Department of Agricultural Biotechnology, Seoul National University)
  • Yong Wang(Department of Agricultural Biotechnology, Seoul National University)
  • Xueying Tao(Department of Agricultural Biotechnology, Seoul National University)
  • Jae Young Choi(Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Jong Bin Park(Department of Agricultural Biotechnology, Seoul National University)
  • Hee Jin Shim(Department of Agricultural Biotechnology, Seoul National University)
  • Soo Dong Woo(Department of Plant Medicine, Chungbuk National University)
  • Byung Rae Jin(College of Natural Resources and Life Science, Dong-A University)
  • Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University)