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Genomic structure of the Spodoptera litura multicapsid nucleopolyhedrovirus

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/291447
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

The complete genomic nucleotide sequence of the Spodoptera litura multicapsid nucleopolyhedrovirus (SlMNPV) isolated in Korea, SlMNPV-K1, was determined. It was 137,435 bp long, with a 55.4 % A+T content and contained 132 putative open reading frames (ORFs) of 150 nucleotides or larger that showed minimal overlap. The 132 putative ORFs covered 87.7% of the genome. Among these, 131 ORFs were are homologous to genes identified in previously reported SlMNPV genome which consisted 139,342 bp and contained 141 putative ORFs. However, arrangement of some ORFs were somewhat different from each other. Even though the SlMNPV-K1 genome is smaller than that of previously reported SlMNPV genome and had lesser predicted ORFs, the main functional genes were all conserved. When the phylogenic relationship was analyzed using the nucleotide sequence of polyhedrin gene, SlMNPV-K1 was most closely related to Lymantria dispar multicapsid nucleopolyhedrovirus (LdMNPV) which were belonged to Group Ⅱ nucleopolyhedrovirus.

저자
  • Yong Wang(Department of Agricultural Biotechnology, Seoul National University)
  • Jae Young Choi(Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Hee Jin Shim(Department of Agricultural Biotechnology, Seoul National University)
  • Jong Yul Roh(Department of Agricultural Biotechnology, Seoul National University)
  • Hong Guang(Department of Agricultural Biotechnology, Seoul National University)
  • Qin Liu(Department of Agricultural Biotechnology, Seoul National University)
  • Soo Dong Woo(College of Agriculture, Life and Environment Sciences, Chungbuk National University)
  • Byung Rae Jin(College of Natural Resources and Life Science, Dong-A University)
  • Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University)