논문 상세보기
pp.19-26
The phytochemicals of many plants suggest their potential use as dietary supplements in cancer chemoprevention and chemotherapy. In the present study, antitumor activity of Cudrania tricuspidata, a plant native to East Asia, was investigated. Cell growth inhibition of the extract on HT-29 colorectal adenocarcinoma using MTT colorimetric assay was determined. Apoptosis on HT-29 cells was performed by DNA fragmentation analysis. PGE2 release was measured by enzyme immunoassay, because PGE2 is a key protumorigenic prostanoid in many human cancers. For the ROS scavenging activity, ROS level was detected by laser scanning confocal microscope. It was found that methanol extract of leaves inhibits cell viability by inducing apoptosis as evidenced by DNA fragmentation. Stem bark decreases synthesis of PGE2, inflammatory mediator. Fruits exhibited pronounced ROS scavenging activity. Taken together, these results suggest that Cudrania tricuspidata exerts growth inhibition and anti-oxidation on HT-29 cells through apoptosis, ROS scavenging respectively that it may have anti-cancer properties.
II. 재료 및 방법
1. 재료
2. 세포배양
3. 세포생존율 분석(MTT assay)
4. 세포고사 확인(DNA 분절 분석)
5. Prostaglandin E2 생합성(효소결합면역측정법)
6. COX-2 유전자 발현(실시간 역전사 중합효소 연쇄반응)
7. 활성산소기 확인(공촛점현미경)
8. 통계 분석
III. 결과
1. 대장암세포주에서 꾸지뽕나무 추출물이 세포성장 억제에 미치는 효과
2. 꾸지뽕나무잎 추출물 처리에 의한 대장암세포주의세포자멸사
3. PGE2 생합성 억제 및 COX-2 유전자 발현
4. 활성산소기 제거
V. 고찰
VI. 참고문헌
