Cyclosporine A (Cs A) which is a highly lipophilic cyclic undecapeptide mainly used for its immunosuppressive properties exert a wide spectrum of biological activities including fungicidal antiproliferactive, anti-inflammatory and chemotherapuetic effects. Human salivary gland adenocarcinoma is very aggressive characteristics, which is need to get the effective chemotherapuetic methods. Subconfluent SGT cell cultures have been treated with CsA at in vivo relevant concentrations for 24h. MTT assay for cellular proliferation of cultured SGT cell line has been performed and TGase 1 activity assay for cellular differentiation has been detected in the CsA-treated samples. It suggested that CsA could have an inhibitory effect in the proliferation of SGT cell line but no in the differentiation.

I. INTRODUCTION
 II. MATERIALS AND METHODS
  1. Cell Culture Condition
  2. Cyclospoin A (CyA) Concentration
  3. MTT (5-diphenyl dimethyl-tetrazoliumbromide) Assay
  4. Growth Curve of SGT
  5. Transglutaminase 1 Assays for Differenceationof SGT Cell Line
 III. RESULTS
 IV. DISCUSSIONS
 V. REFERENCES

• 이종헌(Department of Oral Pathology, the Oral aging Research Center, Dental College, Dankook University) | Chong Heon Lee correspondence