Taraxaci Herba has long been used in herbal medicine for their choleretic, anti-heumatic and diuretic properties. In the present study, we investigated the effects of origin plants of Taraxaci Herba, Taraxacum coreanum Nakai, as an anti-inflammatory agent in lipopolysaccharide(LPS)-induced microglial activation in BV2 cells. NNMBS273, the EtOH extracts of roots T. coreanum was examined for anti-neuronal inflammatory activity as new drug development. The roots of T. coreanum, showed the potent anti-neuroinflammatory effects on LPS-induced inflammation in microglial BV2 cells. The anti-inflammatory effects of NNMBS273, the EtOH extracts of roots T. coreanum was demonstrated by the suppression of pro-inflammatory mediators, including pro-inflammatory enzymes (inducible nitric oxide synthase and cyclooxygenase-2) and cytokines (tumor necrosis factor-α and interleukin-1β). These results suggest that the roots T. coreanum may be a promising candidate for the treatment of neurodegenerative diseases related to neuroinflammation.

Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 실험 재료
  2. 시약 및 기기
  3. 시료의 제조
  4. Western Blot Analysis
  5. Nitrite assay
  6. EIA에 의한 PGE2 측정
  TNF
  8. 통계처리
 Ⅲ. 결과
  1. 흰 민들레 뿌리 EtOH 추출물(NNMBS273)의 iNOS발현 억제 작용
  2. 흰 민들레 뿌리 EtOH 추출물(NNMBS273)의COX-2 발현 억제 작용
  3. 흰 민들레 뿌리 EtOH 추출물 (NNMBS273)의 NO및 PGE2 생성 억제 작용
  4. 흰 민들레 뿌리 EtOH 추출물(NNMBS273)의TNF-α와 IL-1β 생성 억제 작용
 Ⅳ. 고찰
 Ⅴ. 결론

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