Fucoidan has been extensively studied as medicinal materials due to its biological activities including osteoblastic differentiation effect. However, osteoblastic effect by fucoidan is unknown in alveolar bone marrow derived mesenchymal stem cells (ABM-MSCs). The present study was undertaken to evaluate the effect of fucoidan on Osteoblastic differentiation in ABM-MSCs and explore its mechanism. Cell proliferation was analyzed by crystal violet staining. Osteoblast differentiation was determined by alkaline phosphatase activity, calcium accumulation assay and gene expression of osteoblast markers. We found that fucoidan induced cell proliferation of ABM-MSCs. Furthermore, fucoidan increased the ALP activity, calcium accumulation, and osteoblast specific genes such as Runx2, type I collagen alpha 1. Moreover, fucoidan induces the expression of asporin and bone morphogenic protein (BMP)-2 and asporin. Based on these results, these finding indicate that fucoidan induces osteoblast differentiation in ABM-MSCs and partially enhanced the mRNA expression of BMP-2 and asporin.

Ⅰ. INTRODUCTION
 Ⅱ. MATERIALS AND METHODS
  1. 중간엽 줄기세포의 배양
  2. 세포 증식 분석
  3. Alkaline phosphatase (ALP) 염색과 활성 측정
  4. 칼슘 축적 분석
  5. Reverse Transcription-polymerase Chain Reaction(RT-PCR
  6. 통계처리
 Ⅲ. RESULTS
  1. 후코이단에 의한 세포 증식
  2. 후코이단이 ALP 활성에 미치는 영향
  3. 후코이단이 칼슘축적에 미치는 영향
  4. 후코이단에 의한 골 분화 표식 유전자 발현 확인
  5. 후코이단에 의한 골 형성 분화 과정에서 나타난 유전자 발현
 IV. DISCUSSION
 Ⅵ. REFERENCES

• 김범수(원광골재생연구소, ㈜본셀바이오텍) | Beom Su Kim
• 강효진(원광골재생연구소) | Hyo Jin Kang
• 박지윤(㈜본셀바이오텍) | Ji Yun Park
• 양선식(원광골재생연구소) | Sun Sik Yang
• 윤정훈(원광골재생연구소, 원광대학교 대전치과병원 구강병리과) | Jung Hoon Yoon
• 이준(원광골재생연구소, 원광대학교 대전치과병원 구강악안면외과) | Jun Lee correspondence