Doenjang is one of the most favorite traditional food which was produced by the fermentation process of soybean with various microbes such as Aspergillus oryzae, Bacillus subtilis and so on. The aim of this research is to analyse the antioxidant and anti-α-glucosidase inhibitory activities of doenjang made by 9 different cultivar (Daewonkong, Taekwangkong, Daepungkong, Hwangkeumkong, Saedanbaek, Hanolkong, Pungsannamulkong, Cheongja-3 and Cheongdu-1). Doenjang samples were made by traditional manner using rice straw. Each samples were extracted with 40% methanol and extract were used Among them, cheongja-3‘s doenjang showed a significant highest antioxidant and anti-α-glucosidase inhibitory activities because of its anthocyanin. Except for cheongja-3's Doenjang, antioxidant activity had positive correlation with isoflavone content. But no relationship observed between isoflavone content and anti-α-glucosidase activity. The highest anti-α-glucosidase activity was observed in Saedanbaek's Doenjang with highest protein content.