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Molecular Analysis of Genes Related to Tryptophan Biosynthesis and Grain Quality Evaluation in High Tryptophan Rice
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/297898
Fortification with vitamins in crops like rice is a continuing endeavor for geneticists and rice breeders. Tryptophan is one of the essential amino acids needed in human diet. In this study, we developed rice mutant lines using ethyl methane sulfonate (EMS) treatment in Korean cv. Donganbyeo and candidate rice lines were selected by insensitivity to the tryptophan analog, 5-methyltryptophan. One of the mutants has a 20-25 fold higher tryptophan level in mature seeds than wild type. To identify the mutations in anthranilate synthase genes, OASA1 and OASA2 sequences were generated. Moreover, mRNA expression levels of tryptophan biosynthesis related genes were examined. To further qualify the tryptophan fortification in rice, comparative assessment of cooking and eating quality was conducted with mutant lines and wild type. The moisture, viscosity, taste quality, protein content, amylose content and amino acid composition were similar with wild type. However, tryptophan contents in the mutant lines were higher than wild type as we targeted. The mutation present in AS gene of 5MT resistant rice may prove useful for the generation of crops with increased tryptophan contents and the mutation differences in AS sequences can be used for selection of mutant lines with high tryptophan level from large population.
