Toxic (high phorbol esters) and nontoxic (low phorbol esters) jatropha accessions cannot be distinguished morphologically. Their seeds must be chemically analyzed through a complex and costly process using HPLC method. EST-SSR markers can be used to classify jatropha accessions with high and low phorbol esters. In this study, ninety-seven EST-SSR markers amplified the genomic DNA and showed polymorphism among 5 high phorbol esters accessions and 10 low phorbol esters accessions. These markers can be further exploited for jatropha improvement through marker-assisted breeding.

• Kularb Laosatit(Programin Plant Breeding, Faculty of Agriculture at KamphaengSaen, Kasetsart University)
• Patcharin Tanya(Department of Agronomy, Faculty of Agriculture at KamphaengSaen, Kasetsart University)
• Suk-Ha Lee(Department of Pant Science and Research Institute of Agriculture and Life Sciences, Seoul National University, Plant Genomics and Breeding Institute, Seoul National University)
• Peerasak Srinives(Department of Agronomy, Faculty of Agriculture at KamphaengSaen, Kasetsart University)