Toxic (high phorbol esters) and nontoxic (low phorbol esters) jatropha accessions cannot be distinguished morphologically. Their seeds must be chemically analyzed through a complex and costly process using HPLC method. EST-SSR markers can be used to classify jatropha accessions with high and low phorbol esters. In this study, ninety-seven EST-SSR markers amplified the genomic DNA and showed polymorphism among 5 high phorbol esters accessions and 10 low phorbol esters accessions. These markers can be further exploited for jatropha improvement through marker-assisted breeding.

Mungbean (Vigna radiata) is a fast-growing, warm-season legume crop that is primarily cultivated in developing countries of Asia. We constructed a draft genome sequence of mungbean to facilitate genome research into the subgenus Ceratotropis and to enable a better understanding of the evolution of leguminous species. The draft genome sequence covers 80% of the estimated genome, of which 50.1% consists of repetitive sequences. In total, 22,427 high confidence protein-coding genes were predicted. Based on the de novo assembly of additional wild mungbean species, the divergence of what was eventually domesticated and the sampled wild mungbean species appears to have predated domestication. Moreover, the de novo assembly of a tetraploid Vigna species (Vigna reflexo-pilosa var. glabra) provided genomic evidence of a recent allopolyploid event. To further study speciation, we compared de novo RNA-seq assemblies of 22 accessions of 18 Vigna species and protein sets of Glycine max and Cajanus cajan. The species tree was constructed by a Bayesian Markov chain Monte Carlo method using highly confident orthologs shared by all 24 accessions. The present assembly of V. radiata var. radiata will facilitate genome research and accelerate molecular breeding of the subgenus Ceratotropis.

Grain quality and days to flowering are major characters to be considered in vegetable soybean improvement. Theobjective of this study was to investigate the inheritance of both traits in recombinant inbred lines (RILs) derived from a crossenvironments. T

An experiment was conducted to determine N₂-fixation efficiency using 5 soybean genotypes, viz. 'Chiang Mai (CM) 60', 'SJ1', 'Santa Maria', 'KUSL 20004', 'AGS 292' and 40 rhizobium strains from the Soil Microbiology Group, Soil Science Division