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Identification of the Nicotinic Acetylcholine Receptorsfrom the Phyllotreta striolata (Coleoptera: Chrysomelidae)
The striped fleas beetle (SFB), Phyllotreta striolata is a notorious and widely distributed pest in the world including Korea. We surveyed SFB insecticide resistance in Pyeongchang and Hoengseng population. Leaf dipping bioassay with recommended concentration of insecticides was performed using SFB adults. Two populations showed high level of resistance in various insecticides particularly neonicotinoids. Nicotinic acetylcholinesterase receptor (nAChR) is well known target site in neonicotinoid. Therefore, we tried to identify the nAChR from the SFB. Primary, partial contig sequence obtained by RNAseq from Hoengseng population and additional gap fillings were conducted. Total 12 nAChR subunits (10 alpha subunits and 2 beta subunits) were identified and phylogenetic relationships were predicted. Earlier studies reported that point mutations contribute to develop the imidacloprid resistance in aphids and brown planthopper, however we did not find it. Further studies relative to neonicotinoid insecticide resistance mechanism are demanded.
