The study was conducted on the effects of time of defoliation on fruit quality of pear (Pyrus pyrifolia Nakai) trees, managing with low-pesticides, and regrowth of stem cuttings in vitro. Treatments included for 40% of uniform defoliation at early-August, end-August, and early-September, as well as control (no defoliation). Defoliation at early-September and control increased growth of water sprouts as well as concentrations of carbohydrates, total nitrogen, and free sugar in one-year old shoots. Defoliation at early-September and control increased fruit yield and mean fruit weight, with high soluble solids content and fruit surface color of a* observed for both defoliation at end-August and early-September. Defoliation at early-August increased rates of electrolyte leakage in stem cuttings at -18℃ in vitro. There were no significantly different for germination rates of the cuttings between the treatments at -18 and -21℃ in vitro, with the highest germination of the cuttings observed for defoliation at early-September and control at -27℃. Therefore, orchard management should be performed to be minimized for defolia-tion of the spur leaves until end-August, causing from precipitation and pests.