This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and 35oC, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and MgSO4·7H2O and FeSO4·7H2O as mineral salts.

Introduction
 Materials and Methods
  Fungal isolates
  Culture media
  Temperature
  Effect of pH
  Effect of favorable nutrient sources
 Results and Discussion
  Screening of the suitable culture media
 Acknowledgement
 Reference

• Woo-Sik Jo(Gyeongbuk Province Agricultural Technology Administration, Daegu 702-708, Korea) Corresponding author
• Ju-Ri Park(Gyeongbuk Province Agricultural Technology Administration, Daegu 702-708, Korea)
• So-Ra Oh(Gyeongbuk Province Agricultural Technology Administration, Daegu 702-708, Korea)
• Min-Gu Kang(Gyeongbuk Province Agricultural Technology Administration, Daegu 702-708, Korea)
• Woo-Hyun Kim(Gyeongbuk Province Agricultural Technology Administration, Daegu 702-708, Korea)
• Seung-Chun Park(College of Veterinary medicine Kyugpook National University, Daegu 702-701, Korea)