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전자선 처리에 따른 마른 김(Porphyra tenera)의 미생물 저감화 효과와 저항성 세균의 동정 KCI 등재 SCOPUS

Identification of electron beam-resistant bacteria in the microbial reduction of dried laver (Porphyra tenera) subjected to electron beam treatment

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한국식품저장유통학회 (The Korean Society of Food Preservation)
초록

This study investigated the effect of electron beam (EB) treatment on the microbial reduction of dried laver (Porphyra tenera) and identified EB-resistant bacteria from the treated dried laver. After EB treatments of 4 kGy and 7 kGy, the numbers of total bacteria and EB-resistant bacteria were measured using tryptic soy agar and mannitol salt agar, respectively. The morphological and biochemical characteristics of each isolated EB-resistant bacteria were investigated and these bacteria were identified. Compared to the control (1.5±0.2)×106 CFU/g, the total bacterial number was significantly decreased to (5.4±0.5)×104 CFU/g and (1.1±0.6)×104 CFU/g after EB treatments of 4 kGy and 7 kGy, respectively. With a higher EB dosage, the number of red colonies was almost same, whereas the number of yellow colonies was significantly decreased to (3.3±1.2)×103 CFU/g and 0 CFU/g for 4 kGy and 7 kGy, respectively. All red and yellow colonies were gram-positive cocci, catalase-positive, and resistant to 3% and 5% NaCl media. From the 16S rDNA sequence analysis, yellow and red colonies were identified as either Micrococcus flavus or M. luteus, with 99% similarity for the yellow colonies, and Deinococcus proteolyticus and D. piscis, with 99% and 97% similarity for the red colonies, respectively.

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저자
  • 김유진(경북대학교 식품공학부) | You Jin Kim
  • 오희수(경북대학교 응용생명과학부) | Hui Su Oh
  • 김민지(경북대학교 식품공학부) | Min Ji Kim
  • 김정훈(경북대학교 응용생명과학부) | Jeong Hoon Kim
  • 고재백(경북대학교 응용생명과학부) | Jae Baek Goh
  • 최인영(경북대학교 식품공학부) | In Young Choi
  • 박미경(경북대학교 식품공학부) | Mi-Kyung Park Corresponding author