The aim of this study was to investigate the method validation for the determination of loganin content in Cornus officinalis (CO). This medicinal plant reportedly mainly included loganin. The specificity, linearity, precision, accuracy, limit of detection (LOD), and quantification (LOQ) were measured by HPLC/DAD. Our results showed that high linearity in the coefficient of calibration correlation (R2) for loganin standard was 1. LOD and LOQ for loganin were 0.007 and 0.026 mg/mL, respectively. The recovery rate of loganin was revealed to be in the high range of 95.14-105.45%. The relative standard deviation of intra- and inter-day precision of loganin in CO was 1.8% and 2.3%, respectively. The loganin content of CO from Gurye, Uiseng, Ichoen, and China were 7.81, 3.41, 7.42, and 6.52 mg/g, respectively. In conclusion, these methods were validated for the detection of loganin in CO.

서 론
 재료 및 방법
  실험재료
  표준용액의 조제
  시료 추출 및 HPLC 분석
  분석법 검증
  통계분석
 결과 및 고찰
  특이성 확인
  직선성, 검출한계 및 정량한계
  정확성 및 정밀성
  산수유 중 loganin 함량
 요 약
 감사의 글
 참고문헌

• 장다은(국립농업과학원 기능성식품과) | Da eun Jang
• 이상훈(국립농업과학원 기능성식품과) | Sang Hoon Lee
• 황인국(국립농업과학원 기능성식품과) | In Guk Hwang
• 송진(국립농업과학원 기능성식품과) | Jin Song
• 황경아(국립농업과학원 기능성식품과) | Kyung-A Hwang Corresponding author