In this study, to improve the in vitro development of various cells including cloned embryos, the effects that isoproterenol and melatonin have on in vitro development of porcine parthenogenetic oocytes were investigated. Parthenogenetic activation was induced with electrical stimulation, BSA and 6-DMAP treatment. 10-7 M of melatonin and isoproterenol (10-10, 10-12 and 10-14 M) were supplemented for in vitro maturation (IVM) and in vitro culture (IVC) medium, with different concentrations. When isoproterenol and melatonin were supplemented in IVM medium with different concentrations, there was no significant (P<0.05) difference of maturation rate in the treatment groups as well as in that of only melatonin. As isoproterenol and melatonin were supplemented in IVM medium with different concentrations, blastocyst rates of isoproterenol 10-12 M treatment group (37.1%) were significantly (P<0.05) higher than control group (26.0%). Isoproterenol and melatonin were supplemented in IVC medium with different concentrations, then the cleavage rate of 10-12 M isoproterenol treatment group (82.2%) was significantly (P<0.05) higher than the group that melatonin was only supplemented (70.9%). There was no difference of blastocyst rate between the treatment groups. When isoproterenol and melatonin were supplemented for IVM+IVC medium with different concentrations, the cleavage rate of 10-12 M isoproterenol treatment group (92.5%) was significantly (P<0.05) higher than the control group (82.8%) and the group that melatonin was only treated (81.6%). The blastocyst rate of 10-12 M as 45.6% was significantly (P<0.05) higher than control group (25.2%) and melatonin treatment group (31.2%). The cell number of blastocyst in 10-12 M isoproterenol treatment group 35.5±3.4 was significantly (P<0.05) highest. The results of this study showed that the development rate of IVC when both isoproterenol and melatonin were supplemented was higher than when melatonin was only supplemented. Therefore, it is concluded that isoproterenol is rather effective in the activation of melatonin. 10-7 M melatonin and 10-12 M isoproterenol were considered suitable concentration.

서 론
 재료 및 방법
  1. 난포란의 채란 및 체외 성숙
  2. 성숙 난자의 단위 발생 유기
  3. 단위발생란의 체외 배양 및 핵 수 조사
  4. 실험 설계
  5. 통계학적 분석
 결 과
  1. 돼지 난포란의 체외 성숙에 있어서 Isoproterenol과 Melatonin의 첨가 농도에 따른 체외 성숙율
  2. 돼지 난포란의 체외 성숙에 있어서 Isoproterenol과 Melatonin의 첨가 농도에 따른 단위발생란의 체외 발달율
  3. 돼지 단위발생란의 체외 배양에 있어서 Isoproterenol과 Melatonin의첨가 농도에 따른 체외 발달율
  4. 돼지 난포란의 체외 성숙과 배양시 Isoproterenol과 Melatonin의 첨가 농도에 따른 단위발생란의 체외 발달율
 고 찰
 결 론
 REFERENCES

• 정다운(경남과학기술대학교 동물생명과학과) | Da-Un Jeong
• 윤윤진(경남과학기술대학교 동물생명과학과) | Yun-Jin Yun
• 박희성(경남과학기술대학교 동물생명과학과) | Hee-Sung Park orresponding author