논문 상세보기
pp.27-32
This study was conducted to evaluate the effects of different volume (100 μl vs. 2 ml) of microdrop culture on B6D2F1 mice oogenesis. In the present study, B6D2F1/CrljOri F1 mice were utilized in order to maximize oogenesis. Also we used TCM-199, Dulbecco's medified Eagle's medium (DMEM), embryo culture medium (Fertilization medium, Cleavage medium, Blastocyst medium), G series medium and One step medium. Blastulation rate was not different between groups (58.4±2.9% vs. 61.2±4.8%). Zona hatched rate (38±15.4% vs. 27±3.4%) and attached rate (55±13.9% vs. 46±3.9%) did not differ by the volume of culture media. Total cell numbers (59.8±9.7 vs. 70.3±8.7), ICM cell numbers (15.8±0.6 vs. 16.8±1.5), TE cell numbers (44.0±9.7 vs. 53.6±7.3), % ICM (26.4±2.9% vs. 23.8±3.3%) and ICM:TE ratio (1: 2.8±0.4 vs. 1: 3.2±0.6) were not different between groups (i.e., 100 μl vs. 2 ml). These results show that the capacity of the culture medium did not effect the cell numbers of B6D2F1 mice blastocysts. In summary, these results can provide fundamental data to maximize culture condition for in vitro fertilization on B6D2F1 mice.
재료 및 방법
1. 실험동물
2. B6D2F1 마우스 배양액
3. 마우스 난자의 준비
4. 체외 성숙(In Vitro Maturation, IVM)
5. 체외수정(In Vitro Fertilization, IVF)
6. 배아 배양(In Vitro Culture, IVC) 및 포배아의 이중형광염색
7. 통계처리
결 과
1. 배양액 용량(100 μl와 2 ml)이 B6D2F1 마우스 2세포기 배아의 포배아 형성률에 미치는 영향
2. 배양액 용량(100 μl와 2 ml)이 B6D2F1 마우스 포배아 세포수에 미치는 영향
3. 배양액 용량(100 μl와 2 ml)이 B6D2F1 마우스 포배아의 투명대 탈출과 착상률에 미치는 영향
고 찰
결 론
REFERENCES
