This study was conducted to evaluate the effects of type of culture media (BM, G2, OS, TCM, and MEM) on B6D2F1 mice oogenesis. In the present study, B6D2F1/CrljOri F1 mice were utilized in order to maximize oogenesis. Also we used TCM-199, Dulbecco's medified Eagle's medium (DMEM), embryo culture medium (Fertilization medium, Cleavage medium, Blastocyst medium), G series medium and One step medium. In vitro maturation was highest in BM followed by the order of OS, MEM, TCM and G2 (90±2.8% > 88±3.2% > 85±4.9% > 78±10.2% > 64±7.7%, respectively). To note, the G2 group was statistically different compared to other groups (p<0.05). On the other hand the fertilization rate was highest in the G2 group followed by BM, OS, TCM, and MEM (87±7.2% > 85±6.9% > 74±14.0% > 71±13.8% > 2±1.4%, respectively). The MEM group was significantly lower compared to other groups (p<0.05). The developmental rate was highest in the OS group followed by the G2 group and the BM group albeit no statistical significance was noted (73±11.6% > 71±9.2% > 66±10.4%). Of note, all cells of the TCM and MEM groups were died during embryonic development. The zona hatched rate (51±9.8% vs. 50±9.1% vs. 47±7.2% for BM, G2, and OS respectively) and attached rate (45±12.3% vs. 38±16.1% vs. 37±11.5% for BM, G2, and OS respectively) were not different amongst groups. No difference was found in total cell numbers (74±13.9 vs. 64±9.2 vs. 76±6.7 for BM, G2, and OS respectively), ICM cell numbers (20±1.9 vs. 14±1.8 vs. 15±2.1), TE cell numbers (55±12.5 vs. 49±10.7 vs. 61±5.9), % ICM (30±2.8% vs. 24±7.0% vs. 22.8±2.2%) and ICM:TE ratio (1:2±0.5 vs. 1:3.1±0.8 vs. 1:3.1 ±0.5) amongst groups. In summary, these results can provide fundamental data to maximize culture condition for in vitro fertilization on B6D2F1 mice.

서 론
 재료 및 방법
  1. 실험동물
  2. B6D2F1 마우스 배양액
  3. 마우스 난자의 준비
  4. 체외 성숙(In Vitro Maturation, IVM)
  5. 체외수정(In Vitro Fertilization, IVF)
  6. 배아 배양(In Vitro Culture, IVC) 및 포배아의 이중형광염색
  7. 통계처리
 결 과
  1. 서로 다른 배양액(BM, G2, OS, TCM과 MEM)이 B6D2F1 마우스 난자의 성숙․수정율, 포배아 형성율에 미치는 영향
  2. 서로 다른 배양액 종류가 B6D2F1 마우스 포배아의 세포수에 미치는 영향
  3. 서로 다른 배양액 종류가 B6D2F1 마우스 포배아의 투명대 탈출과 착상율에 미치는 영향
 고 찰
 결 론
 REFERENCES

• 유창석(대구대학교 동물자원학과) | Chang-Seok Yoo
• 박기상(대구대학교 동물자원학과, 경북대학병원 산부인과) | Kee Sang Park
• 서병부(대구대학교 동물자원학과, 대구대학교 생명환경연구소) | Byoung Boo Seo Corresponding author