Background: In the herbal medicine market, Angelica gigas, Angelica sinensis, and Angelica acutiloba are all called "Danggui" and used confusingly. We aimed to assess the genetic diversity and relationships among 14 Angelica species collected from different global seed companies. Toward this aim we developed DNA markers to differentiate the Angelica species.
Methods and Results: A total of 14 Angelica species, A. gigas, A. acutiloba, A. sinensis, A. pachycarpa, A. hendersonii, A. arguta, A. keiskei, A. atropurpurea, A. dahurica, A. genuflexa, A. tenuissima, A. archangelica, A. taiwaniana, and A. hispanica were collected. The genetic diversity of all 14 species was analyzed by using five chloroplast DNA-based simple sequence repeat (SSR) markers and employing the DNA fragment analysis method. Each primer amplified 3 - 12 bands, with an average of 6.6 bands. Based on the genetic diversity analysis, these species were classified into specific species groups. The cluster dendrogram showed that the similarity coefficients ranged from 0.77 to 1.00.
Conclusions: These findings could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Angelica species. The analysis of polymorphic SSRs could provide an important experimental tool for examining a range of issues in plant genetics.

서 언
 재료 및 방법
  1. 시료 수집 및 DNA 추출
  2. 엽록체 기반 SSR 마커 탐색
  3. 유전적 다양성 및 유연관계 분석
 결과 및 고찰
  1. 당귀 엽록체 DNA분석을 통한 SSR 마커 개발
  2. 계통도 작성을 통한 14종 당귀의 유연관계 분석
 REFERENCES

• 박상익(충북대학교 농업생명환경대학 특용식물학과) | Sang Ik Park
• 김세림(충북대학교 농업생명환경대학 특용식물학과) | Serim Kim
• 길진수(충북대학교 농업생명환경대학 특용식물학과) | Jinsu Gil
• 이이(충북대학교 농업생명환경대학 특용식물학과) | Yi Lee
• 김호방((주)바이오메딕 생명과학연구소) | Ho Bang Kim
• 이정호((주)녹색식물연구소) | Jung Ho Lee
• 김성철(농촌진흥청 국립원예특작과학원 인삼특작부 약용작물과) | Seong Cheol Kim
• 정찬식(농촌진흥청 국립원예특작과학원 인삼특작부 약용작물과) | Chan Sik Jung
• 엄유리(국립산림과학원 산림약용자원연구소) | Yurry Um Corresponding author