Background: This study aimed to investigate the antioxidat and antimicrobial activities of the methanol extract and its fractions prepared from the roots of Sanguisorba officinalis L.
Methods and Results: The antioxidant activities were compared by evaluating the DPPH radical and nitric oxide (NO) scavenging ability. Measurement of DPPH radical scavenging ability showed that the SC50 values of the ethyl acetate fraction was 3.85 ㎍/㎖. The ethyl acetate fraction exhibited the most effective DPPH radical scavenging ability compared with the other samples. As for the NO scavenging ability, at all tested concentrations, the ethyl acetate fraction showed a higher scavenging activity than that of the extract and other fractions. These results are related to the total phenolic compound and flavonoid contents of the ethyl acetate fraction. Antimicrobial activity against foodborne pathogens was investigated using the disc diffusion assay. The ethyl acetate fraction showed the highest antimicrobial activity against gram-positive Staphylococcus aureus and Bacillus cereus. However, the chloroform fraction had a higher antimicrobial activity against gram-negative Vibrio vulnificus than that of the extract and other fractions.
Conclusions: The results show that the ethyl acetate fraction had a higher antioxidant as well as antimicrobial activity, than did the other samples. Therefore, the ethyl acetate fraction has potential application in the food industry.

서 언
 재료 및 방법
  1. 재료
  2. 추출 및 분획
  3. 총 polyphenol 및 flavonoid 함량 측정
  4. DPPH radical 소거능 측정
  5. Nitric oxide 소거능 측정
  6. Disc diffusion assay에 의한 항균활성 측정
  7. 통계분석
 결과 및 고찰
  1. Total polyphenol 및 flavonoid 함량
  2. DPPH radical 소거능
  3. Nitric oxide 소거능
  4. 추출물 및 분획물의 식중독 원인균에 대한 항균활성
 REFERENCES

• 서고은(동신대학교 생물자원산업화지원센터) | Go Eun Seo
• 김선민(동신대학교 한약재산업학과) | Sun Min Kim
• 표병식(동신대학교 한약재산업학과) | Byoung Sik Pyo
• 양선아(동신대학교 생물자원산업화지원센터) | Sun A Yang Corresponding author