논문 상세보기
pp.271-276
Background: In the present study, we established an HPLC (high performance liquid chromatography)-analysis method for the determination of marker compounds as a part of the material standardization for the development of health-functional foods from Salvia plebeia R. Br. extract.
Methods and Results: The quantitative determination method of hispidulin as a marker compound was optimized by HPLC analysis using a YMC hydrosphere C18 column with a gradient elution system. This method was validated using specificity, linearity, accuracy, and precision tests. It showed a high linearity in the calibration curve with a coefficient of correlation (r2) of 0.999995. The method was fully validated, and was sensitive, with the limit of detection (LOD) at 0.09 ㎍• ㎖−1 and limit of quantification (LOQ) at 0.27 ㎍• ㎖−1. The relative standard deviation (RSD) values of the data from intra- and inter-day precision were 0.05 - 0.22% and 0.32 - 0.42%, respectively, and the intra- and inter-day accuracy of hispidulin were 99.5 - 102.3% and 98.8 - 101.5%, respectively. The average content of hispidulin in Salvia plebeia R. Br. extract was 3.945 ㎎• g−1 (0.39%).
Conclusions: These results suggest that the developed HPLC method is very efficient, and that it could contribute to the quality control of Salvia plebeia R. Br. extracts as a functional ingredient in health functional foods.
재료 및 방법
1. 시약 및 기기
2. 표준용액의 조제
3. 배암차즈기 추출물 분석 시료
4. HPLC 분석조건
5. 정량분석법 검증 (Method validation)
6. 배암차즈기 추출물의 지표성분 hispidulin 분석
7. 통계분석
결과 및 고찰
1. 특이성 확인
2. 직선성 및 범위, 검출한계 정량한계
3. 정확성, 정밀성 및 회수율
4. 배암차즈기 추출물의 Hispidulin 함량
REFERENCES
