A viral genome was assembled de novo from next-generation sequencing (NGS) data from bean bugs, Riptortus pedestris, infected with an entomopathogenic fungus, Beauveria bassiana (Bb), and was further confirmed via the RACE method. Based on the phylogenetic analysis of the RdRp sequences, RiPV-1 was clustered in the unassigned insect RNA viruses with two other viruses, APV and KFV. These three viruses were suggested to constitute a new group of insect RNA viruses. Interestingly, RiPV-1 replication was increased dramatically in bean bugs 2 to 6 days after fungal infection. In conclusion, a novel insect RNA virus was found by NGS data assembly. This virus can provide further insight into the interaction between virus, fungus and the host.

• Se Jin Lee(Department of Agricultural Biology, Chonbuk National University, Korea)
• Yi-Ting Yang(Department of Agricultural Biology, Chonbuk National University, Korea)
• Yu-Shin Nai(Department of Agricultural Biology, Chonbuk National University, Korea, Department of Biotechnology and Animal Science, National Ilan University, Taiwan)
• Mi Rong Lee(Department of Agricultural Biology, Chonbuk National University, Korea)
• Sihyeon Kim(Department of Agricultural Biology, Chonbuk National University, Korea)
• Jae Su Kim(Department of Agricultural Biology, Chonbuk National University, Korea, Plant Medical Research Center, Chonbuk National University, Korea)