본 연구는 향기가 강한 장미를 선발하고 이화학적 향기 특성 을 평가하기 위해 수행되었다. 향기가 있는 장미 라인의 꽃 향기 는 향분석기와 MOS타입 전자코를 이용하여 향 강도와 패턴을 조사하였다. 향기 분석 결과, 장미 라인들 중 ‘RosaScentNIHHS1’ 이 가장 높은 향 강도를 나타냈으며, 전자코 데이터의 주성분분 석(PCA)과 판별함수분석(DFA) 결과, 다른 계통과 뚜렷이 구분 되는 향기 패턴을 보였다. 또한 ‘RosaScentNIHHS1’과 모부본 품종인 ‘위스퍼’ 꽃잎의 휘발성 화합물을 헤드스페이스-고체상미 세추출법(HS-SPME)과 가스크로마토그래피-질량분석기(GC-MS) 를 이용하여 분석하였다. ‘RosaScentNIHHS1’의 주요 휘발성 화합물은 3,5-dimethoxytoluene, citronellol, geraniol이었 으며, 모부본 품종 ‘위스퍼’의 주요 화합물은 3,5-dimethoxytoluene 및 cis-3-hexenyl acetate, 4-vinyl anisole이었다. 두 계통 은 화합물 조성과 각 성분의 상대 성분비에서 상당한 차이를 나타냈다. 본 연구 결과는 향기 특성을 기반으로 한 천연 화훼 자원 개발을 위한 기초 자료로 활용될 수 있을 것이다.

본 연구는 고온기 포인세티아 ‘플레임’의 안정적 재배를 위한 주간온도 관리기준을 제시하고자 수행되었다. 야간 온도를 26℃로 고정한 야간 고온 조건에서 주간 온도를 30℃, 33℃, 36℃, 39℃로 7주간 처리하였다. 39/26℃ 처리구는 5일 이내 전 개체가 고사하였고, 36/26℃ 처리구의 생존율은 60%로 감 소하였다. 초장, 초폭, 분지수는 30/26℃와 33/26℃ 처리구 간에 유의적인 차이가 없었으나, 36/26℃ 처리구는 30/26℃에 비해 각각 29.5%, 30.9%, 27.5% 감소하였다. 신엽 발생은 온도 상승에 따라 급격히 줄어들어 36/26℃에서는 평균 2.3 개로 거의 발생하지 않았다. 27/20℃에 비해 고온처리구에서 엽장은 20.3%~37.6%, 엽폭은 56.4%~57.1% 감소하여 엽장/ 엽폭 비가 증가하였다. F v/Fm과 NDVI는 처리간 큰 차이가 없었 으나, ELP는 36/26℃에서 38%로 증가하였다. 광합성 속도는 27/20℃에서 가장 높았고, 30/26℃와 33/26℃는 유사하였으 나 36/26℃ 처리구는 1,000μmol·m-2·s-1의 광도에서도 0에 가까웠다. 또한 36/26℃에서 증산속도와 세포간 CO₂ 농도가 유의적으로 높게 나타났다. 따라서 주간 33℃ 이상에서 포인세 티아 ‘플레임’은 생리적･형태적 장해가 급격히 증가할 수 있으 므로, 고온기 안정적인 재배를 위해서는 주간 온도를 최소한 33℃ 이하로 관리할 필요가 있을 것으로 판단된다.

Maize (Zea mays. L) is one of the major sources of green fodder for livestock in Pakistan. Crop management plays a key role in obtaining high yields for green fodder. Fertilizer application, seed rate, and row spacing are critical components of crop management, which can significantly affect crop biomass. To determine the best production technology, a two-year (2021-2023) study was conducted at the research area of National Agricultural Research Center, Islamabad. Plant height, number of leaves, leaf area, green fodder yield per acre, and green fodder yield per hectare were recorded. Various row spacing (15 cm, 30 cm, 45 cm, and 60 cm), fertilizer ratio (N: P = 55:30, 65:40, 75:50, and 85:60), and seed rates (30 kg/ac, 35 kg/ac, 40 kg/ac, and 45 kg/ac) were applied. Results obtained experiments revealed that in both growing seasons, the maximum green fodder yield was obtained when fertilizer N: P ratio was 75:50 (green fodder biomass: 74.61 t/ha and 72.56 t/ha). Similarly, the optimal seed rate was found to be 40 kg/ac, which resulted in the highest green fodder yield (73.41 t/ha and 72.88 t/ha in two seasons). Furthermore, the plant of maize at row spacing of 30 cm was found to generate the maximum green fodder yield (72.39 t/ha and 72.40 t/ha, respectively). Green fodder yield per hectare was found to be positively correlated with plant height, number of leaves, and leaf area. These findings underscore the significance of applying a fertilizer ratio of N: P = 75:50, a seed rate 40 kg/ac, and a row spacing of 45 cm for higher yields of green fodder in maize crop.

Production technology trials for PARC’s new fodder oat cultivar (PARC-Oat) were conducted at the National Agricultural Research Center (NARC) under rain-fed conditions in Islamabad from 2021 to 2023. The effects of different fertilizer doses, planting densities (seed rates), and inter-row spacing on green fodder yield were studied. The experiment comprised four fertilizer doses of nitrogen and phosphorus (N:P) (55:30, 65:40, 75:50, and 85:60 kg/ha), four seed rate densities (30 kg/ac, 35 kg/ac, 40 kg/ac, and 45 kg/ac), and four inter-row spacings (15 cm, 30 cm, 45 cm, and 60 cm). Results based o n k ey p arameters a ffecting t he y ield of PARC-O at—namely plant height (cm), leaf area (cm²), leaves per tiller, number of tillers per plant, and green fodder yield (t/ha)—indicated that the maximum yield of 72.74 t/ha was observed with the fertilizer dose of 75:50 kg/ha (N:P). Similarly, a seed rate of 40 kg/ha produced optimal planting densities, resulting in the highest green fodder yield of 72.85 t/ha, while an inter-row spacing of 30 cm yielded the maximum green fodder yield of 74.30 t/ha. These results suggest that to achieve maximum green fodder biomass of oats, best management practices should include the application of a fertilizer dose of 75:50 (N:P), a seed rate of 40 kg/ha, and an inter-row spacing of 30 cm.

In this study, laser-induced graphene oxide (LIGO) was synthesized through a facile liquid-based process involving the introduction of deionized (DI) water onto polyimide (PI) film and subsequent direct laser irradiation using a CO2 laser (λ = 10.6 μm). The synthesized LIGO was then evaluated as a sensing material for monitoring changes in humidity levels. The synthesis conditions were optimized by precisely controlling the laser scribing speed, leading to the synthesis of LIGO with different structural characteristics and varying oxygen contents. The increased number of oxygen-containing functional groups contributed to the hydrophilic properties of LIGO, resulting in a superior humidity sensing capabilities compared with laser-induced graphene (LIG). The LIGO-based sensors outperformed LIG-based sensors, demonstrating approximately tenfold higher sensing responsivity when detecting changes at each humidity level, along with 1.25 to 1.75 times faster response/recovery times, making LIGO-based sensors more promising for humidity-monitoring applications. This study demonstrated laser ablation in a renewable and natural precursor as an eco-friendly and energy-efficient approach to directly synthesize LIGO with controllable oxidation levels.

Islands often have relatively well-preserved ecosystem and an abundance of bioresources with a high conservation value, with unrecorded species continuing to be reported (Hong, 2011). Approximately 1,000,000 species of insect known worldwide (Costello et al., 2012), and 20,710 species are known in Korea (NIBR, 2023). Among these, there are 6,117 species in Korean islands (HNIBR, 2022). Native insect Bio-scan project for Korean islands is to estimate the number of insect species on Korean islands. We attempted to estimate the number of insect species on Korean islands using Barcode Index Number (BIN), and also found unrecorded species. The samples were collected four times from April to July at five locations in the Amtedo, an island located in Shinan-gun Jeollanam-do. We tried to obtain a minimum of one to usually a maximum of four samples per morphospecies to enable DNA barcoding.

Entomopathogenic fungi serve as eco-friendly alternatives to chemical pesticides. In this study, we investigate the interactions between mosquitoes and Metarhizium anisopliae JEF-157, which showed high insecticidal activity against mosquitoes, by RNA-seq analysis. RNA from mosquitoes was extracted at the median lethal time to identify changes in gene expression. The results showed 580 genes were up-regulated, while 336 genes were down-regulated in fungal treated mosquitoes. Up-regulated genes were related to metabolic and cellular processes such as cytochrome P450, DNA replication, and apoptosis. Down-regulated genes were involved in metabolism pathways such as lysosome, starch and sucrose metabolism, and fatty acid biosynthesis. These results are crucial for elucidating the mechanisms of fungal invasion and interaction in insects, providing insights for future pest management strategies.

Entomopathogenic fungi have been studied to control insect pests as an alternative to chemical insecticides. However, all fungi haven't a high virulence against pests. In this study, we compared the biological characteristics of Metarhizium anisopliae strains. First, we selected four M. anisopliae strains and compared the thermotolerance, conidial productivity, and virulence. For the thermotolerance test, conidial suspensions were exposed to 0, 30, 60, and 90 min at 45 °C. As a result, the conidial germination rates were over 95% when exposed for 0 min but, were 64, 37.7, 6, and 3% when exposed for 30 min at 45°C, respectively. To compare conidial productivity, 200g of millet were used and inoculated with a conidial suspension of 1 ml (1×107 conidia/ml). Conidial productivity was investigated after 14 days. As a result of conducting a virulence test against mealworms using a spray method, differences in virulence between strains were confirmed.

Climate change has led to increased insect pests and pest distribution changes. Traditionally, chemical control using synthetic pesticides has been the main method for pest management, but the emergence of pesticide-resistant pests has become a problem. There is a need to develop new pest control agents to overcome these issues. Entomopathogenic fungi used in pest management have minimal environmental side effects and possess a mechanism of action distinct from that of synthetic pesticides. However, there is a need for the development of technologies to maximize the insecticidal effects of fungi against pests, and expressing and releasing dsRNA within the fungi can preemptively knock out the activation of the insect’s defense system, thereby enhancing the insecticidal effect. Controlling insect defense genes and using entomopathogenic fungi as bio-carriers forms a new pest management strategy. This approach, described as a “microbial insecticide agents development strategy of cassette concept, ” can versatilely modify genes and microbes. It is expected to overcome the limitations of synthetic pesticides.