Many species of braconids play an important role in controlling the populations of insects in agricultural and natural ecosystems, which parasitize pests. Nevertheless, the family Braconidae was scarcely research and DNA barcoding of Braconidae has not been studied in Korea. DNA barcoding can rapidly perform species identification, and classify difficult species in morphological identification. Because most braconids are very small and cryptic in morphology, DNA barcoding is useful in Braconidae taxonomy. In this study, genomic DNA of 280 individual samples were extracted, and, among them, 208 samples were obtained COI (cytochrome c oxidase I) barcode sequences. As a consequence of blast, 48 samples were identified in genus-level, and 51 sample were identified in species-level. Among them, 4 species were revealed as unrecorded in Korea.