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pp.149-156
This research is on the halophilic bacteria inhibiting in solar salterns. It was conducted by measuring the composition of cultivating ground in each step of salt production and targeting solar salterns during 2012 and 2013 in Jeungdo, Shinangun, JeollaNam-do, Korea. In each step of salt production, the succession of the group has been studied using pyrosequencing method and the new halophile variety has been separately identified in solar salterns. The results are as follows; The decomposition ability of protein, lipid, cellulose, and starch, has been experimented with separately identifying 33 bacteria that appeared as dominants in solar salterns. Overall, the bacteria with decomposition capability of all 4 components were Micrococcus luteus, Nocardioides daejeonensis in Actinobacteria group, Halobacillus trueperi whb45 in Firmicutes group, and Aurantimonas coralicida, Lutibacterium anuloederans, Nesiotobacter exalbescens in Alphaproteobacteria group. Analysis in the diversity of bacteria community of diluted solution with concentration of 3%, 6%, 11%, 20%, and 30% has been made using pyrosequencing method. In the Order level, SAR 11 dominated in 3% salt solution, Rhodobacterales group (18.9%) in 6% solution, Micrococcales group showed the highest ratio in 11% and 20% solutions, and Rhodobacterales group was dominant in the solution of 30%. In Phylum level, Proteobacteria group showed dominancy in 3% and 6% salt solutions, Actinobacteria group was dominant in 11% and 20% solutions, and Proteobacteria group was dominant in 30% solution. Among the isolated strains, seven novel strains (Kmb21, Kmb26, Kmb27, Kmb32, Kmb42, Kmb64, and Kmb80) were donate registered to NCBI and KCTC, JCM, each as new variety candidate.
