The aim of this study is to assess the replacement effect of black chokeberry (Aronia melanocarpa) used in red-pepper paste seasoning with vinegar. Red-pepper paste seasoning with aronia and vinegar was prepared by mixing red pepper paste, aronia juice or vinegar, sugar and honey, and then aronia juice (J) was added to 0% (C), 50% (A1) and 100% (A2) by replacing the vinegar. As a result, the brightness of the prepared gochujang was decreased with increasing the level of the aronia juice. Total sugar and salinity of C, A1, and A2 showed a significant increase (p<0.05) in compared with that of aronia juice, 24.42 mg/mL and 1.11%, respectively. Also, pH level showed significantly increase with increasing the content of the juice (p<0.05). However, total acidity was decreased with increasing the level of the juice (p<0.05) and A2 (6.55%) especially was lower than the juice, 8.06%. Meanwhile, the total polyphenols were increased with increasing the content of the juice at gochujang samples (p<0.05) and the polyphenols content of the aronia juice was the highest at all samples (p<0.05). The total anthocyanins were also increased with increasing the level of the juice (p<0.05). Antioxidant activity measured by DPPH and ABTS radical scavenging rate was the highest at the aronia juice (p<0.05). Consequently, the result of antioxidant activity of red-pepper paste seasoning with aronia suggested that aronia can be used as a substitute of vinegar in red-pepper paste seasoning with vinegar.