Agrobacterium tumefaciens-mediated transformation (AtMT) has been widely used for generation of fungal transformants and recently applied to Beauveria bassiana. In this study to comprehend how the AtMT promoter influences on the expression of selection marker (hygromycin B resistance gene; hph), two different Ti-Plasmids were constructed: pCeg (gpdA promoter-based) and pCambia-egfp (CaMV 35S promoter-based). Putative transformants were subjected to the PCR, RT-PCR and qRT-PCR to inspect the T-DNA insertion rate and gene expression level. In conclusion, more than 80% of the colonies succeeded in AtMT transformation and the hph expression level of AtMT/pCeg colonies was higher than that of AtMT/pCambia-egfp colonies. This result can provide useful information on the AtMT of B. bassiana, especially antibiotics susceptibility and promoter-dependant expression level.

• Mi Rong Lee(Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University)
• Yu-Shin Nai(Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University Department of Biotechnology and Animal Science, College of Bioresources, National Ilan University)
• Sihyeon Kim(Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University)
• Se Jin Lee(Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University)
• Jae Su Kim(Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University Plant Medical Research Center, Chonbuk National University)