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Microarray에 의한 정상과 불멸화 구강 각화세포의 유전자 발현비교 KCI 등재

Differential Gene Analysis in Normal vs. HPV Immortalized Human Oral Keratinocytes using by cDNA-Microarray

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  • URLhttps://db.koreascholar.com/Article/Detail/324710
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대한구강악안면병리학회지 (The Korean Journal of Oral and Maxillofacial Pathology)
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Studies to evaluate distribution of markers in normal keratinocyte and their immortalized keratinocyte are appropriate to evaluate the normal and preneoplastic lesion of oral cancers as biochemical and cytochemical changes associate with tumorigenesis being not completely understood. Complementary DNA microarray containing 6000 sequence -verified cDNA elements was used to systematically characterize the variation in gene expression patterns of NHOK cells vs. immortalized keratinocyte by HPV16 E6-E7(IHOK). Examination of gene expression that is 85 clones cDNAs exhibits greater than 2 fold overexpression in NHOK probes relative to IHOK probe, 147 cDNAs reveal greater 2 fold overexpression in IHOK relative to NHOK probe.The high similarity in gene expression (96.5%) between IHOK and NHOK cells suggests that only an additional 232/6720 (3.5%) of the genome is differentially gene activated during HPV16 immoratlized keratinocyte growth and differentiation. Examination of gene expression that differs between NHOK and IHOK cellsapprear to be related to : cell adhesion & recognition, cell cycle regulator, apoptosis, transciption factors, growth factors and therir receptors, cytoskeletal and extracellular matrix proteins, signal transduction modulators and effectors, and miscellaneous. The gene expression of cell recognition factor such as endothelin 1, collagen IV, fibronectin, and SPR1 in IHOK were upregulated. Distinct or duplicated cDNA clones representing the same gene were typically clustered in adjacent rows in the clustered gene map. Therefore the differentially expressed and identified genes should be informative in studying oral epithelial cell carcinogenesis and such studies should foster the research of molecular markers allowing to assess the phenotypeof malignant epithelial tumor.

저자
  • 이선경(원광대학교 치과대학 구강병리학교실) | Sun Kyung Lee
  • 이화정(원광대학교 치과대학 구강병리학교실) | Hwa Jeong Lee
  • 최향순(원광대학교 치과대학 구강병리학교실) | Hyang Sun Choi
  • 유문목(원광대학교 치과대학 구강병리학교실) | Mun Mok Rhu
  • 고한영(원광대학교 치과대학 구강병리학교실) | Han Young Guo
  • 김석호(원광대학교 치과대학 구강병리학교실) | Suk Ho Kim
  • 김성현(원광대학교 치과대학 구강병리학교실) | Sung Hyun Kim
  • 원달호(원광대학교 치과대학 구강병리학교실) | Dal Ho Won
  • 박종대(원광대학교 치과대학 구강병리학교실) | Jong Dae Park
  • 박명희(미국 국립보건원 NIDCR, OPCB) | Myung Hee Park
  • 김은철(원광대학교 치과대학 구강병리학교실) | Eun Cheol Kim