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HSG 세포의 성장 및 세포사멸에서 일산화질소와 iron 항상성에 관한 연구 KCI 등재

The study on nitric oxide & iron homeostasis in regulation of cell growth & death in HSG cell

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대한구강악안면병리학회지 (The Korean Journal of Oral and Maxillofacial Pathology)
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Nitric oxide (NO) has been known to inf1uence cell fate through apoptotic or necrotic cell death. Here, we investigated the role of nitric oxide on the growth and viability of immortalized human salivary gland (HSG) cells 띠 vitro. Treatrnent of HSG with a NO donor, S-nitroso-N-acetyl-DL-penκi1lamine (SNAP), significantly diminished the growth rate of HSG in a concentration dependent manner. However, this retardation of cell비ar growth rate was not corresponded to the apoptotic cell death of HSG cells, because there were no characteristic apopto디c features such as condensation of nuclear chromatin, nuclear fragmentation, and the apoptotic peak of propidium iodide (PI)-stained nuclei by flow cytome띠. 까ùs implies that HSG cells are resistant to NO-mediated 다π。to:잉city. 1n SNAP treated HSG cells, cell cycle analysis revealed that the number of G2/M phase increased markedly, according to while the percentage of cells in GO/Gl and S phases was not significantly affected. Otherwise, high concentrations of SNAP increased both P1 and annexin V positive cells. 1nterestingly, preincubation of HSG cells with iron chelator, deferoxamine (DFO), significantly diminished NO cytotoxicity more than when HSG cells are only incubated with SNAP which su잃.ests the role of iron homeostasis in NO-mediated cell death of HSG cells. 1n addi디。n , treatrnent of HSG cells with SNAP specifically cleaved iron regulatory protein-2 (IRP2) while not affecting 1RP1. Collectively, the mπent results s멍gest that NO has a potential to control HSG cell growth through cell cycle arresting at G2/M phase. 1n addi디on , intracellular iron homeostasis nùght play an important role in regulating cell survival of HSG cells

저자
  • 이선경(원광대학교 치과대학 구강병리학교실) | Sun Kyung Lee
  • 이화정(원광대학교 치과대학 구강병리학교실) | Hwa Jeong Lee
  • 고한영(원광대학교 치과대학 구강병리학교실) | Han Young Guo
  • 김석호(원광대학교 치과대학 구강병리학교실) | Seok Ho Kim
  • 김성현(원광대학교 치과대학 구강병리학교실) | Sung Hyun Kim
  • 원달호(원광대학교 치과대학 구강병리학교실) | Dal Ho Won
  • 박종대(원광대학교 치과대학 구강병리학교실) | Jong Dae Park
  • 전창덕(원광대학교 의과대학 미생물 및 면역학교실) | Chang Duk Jeon
  • 김은철(원광대학교 치과대학 구강병리학교실) | Eun Cheol Kim