본 연구에서 루꼴라(Eruca sativa)에 대한 다양한 생리활성을 조사하여 기능성소재 응용가능성을 검토하였다. 루꼴라 추출물은 B. subtilis, E. coli, C. albicans에 대한 항균활성이 매우 우수하게 나타났으며 특히 그람양성세균 B. subtilis에 대한 항균활성이 높았다. 또한, 인볼루크린 발현에 대한 면역조직화학 분석을 인공피부를 이용하여 분석한 결과 루꼴라 추출물을 처리(50 mg/L)한 경우에 0.1% DMSO로 처리한 대조군에 비하여 인볼루크린 발현이 뚜렷하게 증가하였으며 동일농도로 처리된 합성물질 WY14643과 비교하였을 때에도 비슷한 발현양상을 보여 주었다. 이와 같은 결과로 미루어 볼 때 루꼴라 추출물은 피부염 개선 및 피부장벽 기능을 향상할 수 있는 천연물 기반의 소재로 활용하기 위한 매우 효과적인 재료가 될 수 있다고 사료된다.

Eruca sativa is a rocket plant and a member of the Brassicaceae, which is considered to be an important chemo-preventive plant family. Although Eruca sativa has positive biological effects, the effect of Eruca sativa extract (ES) on improvement of skin barrier function has not been reported. In this study, we investigated the applicability of functional materials by examining a variety of physiological activities of Eruca sativa extract. ES showed anti-microbial activities against Bacillus subtilis, Escherichia coli, and Candida albicans. In particular, antimicrobial activities of ES against B. subtilis was the highest. Additionally, immunohistochemical analysis of protein marker related to keratinocyte differentiation was determined. The treatment by ES (50 mg/L) showed a significant increase of involucrin expression compared with treatment by 0.1% DMSO as a control in skin equivalents, the ES-treated group showed similar level in the expression of involucrin compared to the group treated with the same concentration of WY14643 in EpiDermTM, a three-dimensional model of skin equivalents. These results indicate that ES promotes the expression of protein related to barrier properties of the skin. Therefore, ES may be an effective ingredient for skin barrier improvement.

• 김보라(목원대학교 생의약화장품학부, Division of Biomedicinal Chemistry and Cosmetics, Mokwon University) | Bora Kim
• 김현수(중원대학교 식품공학과, Department of Food science and Technology, Jungwon University) | Hyun-Soo Kim Corresponding author