Background : Angelica gigas is a monocarpic biennial or short lived perennial plant. A. gigas, also called Dang Gui or Korean Angelica, is a major medicinal herb used in Asian countries such as Korea, Japan and China. In Korea, we are using the roots of A. gigas, but, they are using Angelica sinensis in China and Angelica acutiloba in Japan to obtain many active constituents. The biggest problem in the using of A. gigas would be the confusion with A. acutiloba or A. sinensis. These three plants can't be distinguished by appearance. And the constituent ratios of the three plants are different. This confusion can cause an accident or the pharmaceutical effects do not meet the expectations. In this study, we developed chloroplast SSR markers that can distinguish A. gigas, A. acutiloba and A. sinensis. Methods and Results : We collected A. gigas, A. acutiloba and A. sinensis. and extrated DNA using CTAB method. The DNA was diluted to 10 ng/㎕ and kept -20℃. We designed the primer sets using CLC Main Workbench based on chloroplast DNA SSR region of A. gigas. PCR were performed on the three angelica plant samples (in 5 repeat). Conclusion : We made five primer sets from five SSR regions of A. gigas cpDNA. All of the primer sets amplified the amplicon effectively. Two of the 5 primer sets had polymorphism. We can distinguish A. gigas, A. acutiloba, and A. sinensis using the 2 primer sets

• Sangik Park(Department of Industrial Plant Science and Technology, Chungbuk National University) | 박상익
• Jinsu Gil(Department of Industrial Plant Science and Technology, Chungbuk National University) | 길진수
• Yurry Um(National Institute of Horticultural and Herbal Science, RDA) | 엄유리
• Hee Chung(Department of Industrial Plant Science and Technology, Chungbuk National University) | 정 희
• Seong Cheol Kim(National Institute of Horticultural and Herbal Science, RDA) | 김성철
• Yi Lee(Department of Industrial Plant Science and Technology, Chungbuk National University) | 이 이 Corresponding author