Effect of Temperature and Water Content During Cold-Stratification on the Germination Properties of Ginseng Seeds
Background : Upon harvest in the summer, seeds of Panax ginseng are unmatured and need further maturation, dehiscence and cold-stratification, for germination. For the cold-stratification, the seeds should be stored in the cold temperature for 90-100 days, however no further description about the storage condition have been described even though there have been many problems in emergence rate and quality of ginseng in the spring-sowed filed. Methods and Results : Thus here we tested 3 different storage temperature(2℃, -2℃, and – 20℃) in combination of 4 different seed water content(59%, 54%, 31%, and 7%) as cold-stratification condition. After 100 days of storage, seeds were placed on the filter paper after watering with distilled water in the petri dish and incubated at 10℃. Fifty percent of seeds stored at 2℃ with 59% water content had already germinated even in the storage room before germination test. Seeds with 59% and 54% water content stored at 2℃ and –2℃ germinated in a similar rate, but emergence of above ground part was higher in the seeds with 54% water content. Seeds with 31% and 7% water content stored at 2℃ and –2℃ showed low germination rate, because of fail in stratification or death. Seeds stored at –20℃ scored even lower germination rate and fail in emergence of above ground part. Conclusion : Seed water content and temperature during the cold-stratification period of ginseng seeds affected on the seed viability and germination rate, thus control of seed water content and storage temperature might improve the emergence rate of spring-sowed ginseng filed.