This study was conducted to investigate the effects of inoculum and carbon sources on anaerobic digestion characteristics. The treatments were combinations of inoculum (digestate of cattle manure and rumen fluid) with carbon sources (starch, cellulose, and xylan). Anaerobic digestion was performed in triplicate at 37°C for 18 days at 100 rpm. Sampling was performed at 0, 1, 2, 3, 4, 5, 7, 9, 12, 15, and 18 days to measure pH, ammonia-N, volatile solids reduction, the cumulative methane content, and the cumulative methane production. There was a significant difference in methane content depending on the carbon source and there was a significant difference in pH, ammonia-N, methane production, and methane content depending on the inoculum (P < 0.05). The results of methane production were higher in the digestate of cattle manure treatment than in the rumen fluid treatment (P < 0.05). In this study, different digestive patterns depending on the type of carbon source could be used as basic research data to set the hydraulic residence time of anaerobic digestion facilities. In addition, the use of ruminal fluid as an inoculum may help accelerate the hydrolysis and acid production steps.

• 최용준(건국대학교 동물생명과학대학) | Yong Jun Choi (College of Animal Science and Technology, Konkuk University)
• 유정원(건국대학교 동물생명과학대학) | Jeong Won Ryu (College of Animal Science and Technology, Konkuk University)
• 이상락(건국대학교 동물생명과학대학) | Sang Rak Lee (College of Animal Science and Technology, Konkuk University) Corresponding author