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한라돌쩌귀로부터 분리된 Dopaol β-D-glucoside의 신장독성 보호효과 KCI 등재

Protective Effect of Dopaol β-D-glucoside Isolated from East Asian Monk’shood on Cisplatin-Induced Nephrotoxicity

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한국약용작물학회지 (Korean Journal of Medical Crop Science)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background: Cisplatin is one of the most extensively used chemotherapeutic agents for the treatment of cancer, including bladder, and ovarian cancers. However, it has been shown to induce nephrotoxicity, despite being an outstanding anti-cancer drug. In this study, we investigated the protective effect of dopaol β-D-glucoside (dopaol) on cisplatin-induced nephrotoxicity. Methods and Results: To confirm the protective effect of dopaol on cisplatin-induced nephrotoxicity, HK-2 cells were treated with 20 μM cisplatin and 80 μM dopaol. Cisplatin increased apoptosis, caspase-3 activity and mitochondrial dysfunction; however pretreatment with 80 μM dopaol successfully attenuated apoptosis, caspase-3 activity and mitochondrial dysfunction. To evaluate the protective effect dopaol on cisplatin-induced nephrotoxicity in vivo, we used an animal model (balb/c mice, 20 ㎎/㎏, i.p. once/day for 3 day). The results were similar to those obtained using HK-2 cells; renal tubular damage and neutrophilia induced by cisplatin reduced following dopaol injection (10 ㎎/㎏, i.p. once/day for 3 day). Conclusions: These results indicate that dopaol treatment reduced cisplatin-induced nephrotoxicity in vitro and in vivo, and can be used to treat cisplatin-induced nephrotoxicity. However, further studies are required to determine the toxicity high dose dopaol and the signal pathways involved in its mechanism of action in animal models.

저자
  • 노종현(한약진흥재단) | Jong Hyun Nho
  • 정자균(한약진흥재단) | Ja Kyun Jung
  • 정호경(한약진흥재단) | Ho Kyung Jung
  • 장지훈(한약진흥재단) | Ji Hun Jang
  • 정다은(한약진흥재단) | Da Eun Jung
  • 이기호(한약진흥재단) | Ki Ho Lee
  • 김아현(한약진흥재단) | A Hyeon Kim
  • 성태경(한약진흥재단) | Tae Kyoung Sung
  • 박호(한약진흥재단) | Ho Park
  • 조현우(한약진흥재단) | Hyun Woo Cho Corresponding author