Commercial applications of OPU/IVP were to produce embryos and calves from high genetic cows.The aim of this present study was to compare the number of recovered oocytes and cultured in vitro produced embryos from Ovum Pick-up (OPU). OPU derived embryo production was carried out of oocytes by ultrasonographic guided follicular aspiration and then produced in vitro produced blastocysts by IVP culture system. In result, the rate of recovered oocytes was obtained 612 (57.2%) and 451(73.7) G1+G2 grade oocytes. No difference of recovered rate (51.1~62.1%) was seen in six donor. The rate of cleavage and blastocyst development were obtained 320 (70.9%) and 78 (24.4%) that was 3.3±0.4 cleaved embryo and 0.9±0.2 blastocysts per session. Cleavage rate of OPU oocytes in No. 6 donor was 90.6%, significantly (P<0.05) higher than that in the other donors, However, blastocysts was similar (25.8~30.0%). In conclusion, limited numbers of OPU oocytes had competent development when cultured in SOF culture medium

• 조상래(농촌진흥청 국립축산과학원) | Sang-Rae Cho (Hanwoo Research Institute, Pyeongchang, Kangwondo, 232-950, Korea)
• 강성식(농촌진흥청 국립축산과학원) | Sung-Sik Kang (Hanwoo Research Institute, Pyeongchang, Kangwondo, 232-950, Korea)
• 김의형(농촌진흥청 국립축산과학원) | Ui-Hyung Kim (Hanwoo Research Institute, Pyeongchang, Kangwondo, 232-950, Korea)
• 이석동(농촌진흥청 국립축산과학원) | Suk-dong Lee (Hanwoo Research Institute, Pyeongchang, Kangwondo, 232-950, Korea)
• 이명숙(농촌진흥청 국립축산과학원) | Myoung-Sook Lee (Hanwoo Research Institute, Pyeongchang, Kangwondo, 232-950, Korea)
• 양병철(농촌진흥청 국립축산과학원) | Byoung-Chul Yang (Hanwoo Research Institute, Pyeongchang, Kangwondo, 232-950, Korea) Corresponding author