Pyraustinae and Spliomelinae are one of the largest subfamily in the family Crambidae, They are reported more than5,000 species in the world. The majority of them is distributed in tropical regions, also some species discovered intosubtropical and warm temperate areas. Laos has a tropical climate because it is located in Southeast Asia. but Laos properlyhas not studied moths diversity by the present.In the present study, we provided a checklist of the subfamily Pyraustinae and Spilomelinae from Laos, including adultphotographs, distributions.