Amyloid-β protein (Aβ) is known to increase free radical production in neuronal cells, leading to cell death by oxidative stress. The purpose of this study was to evaluate the protective effects of PineXol® on Aβ25-35 induced neuronal cell death. Rat pheochromocytoma (PC-12) cells were pre-treated with 100 μg/mL of PineXol® for 2 h. The cells were exposed to single dose of 30 μM Aβ25-35 for 24 h. Cell death was assessed by a cell count kit-8 (CCK-8) assay, lactate and dehydrogenase (LDH) release assay. An Apoptotic process was analyzed by a protein expression of the Bcl-2 family using western blotting. Cell viability increased in PC-12 cells treated with both Aβ25-35 and PineXol®, compared to the control group. PineXol® induced a decrease of the Bcl-2 protein expression (p<0.05), while Bax and Sod1 increased (p<0.05), indicating attenuation of Aβ25-35 induced apoptosis. These results suggest that PineXol® may be a good candidate for the prevention of Alzheimer’s disease(AD).

서 론
 재료 및 방법
  1. 세포배양
  2. 실험재료
  3. 총 폴리페놀 및 플라보이노이드 함량 측정
  4. 세포 생존율 측정
  5. Lactate dehydrogenase (LDH) 측정
  6. Western blot 분석
  7. 통계처리
 결과 및 고찰
  1. 총 폴리페놀 및 플라보노이드 함량
  2. PineXol®이 PC-12 신경세포의 생존에 미치는 영향
  3. Aβ25-35의 처리에 따른 PC-12 신경세포의 세포 사멸
  4. Aβ 유도된 PC-12 신경세포 독성에 대한 PineXol®의보호효과
  5. Aβ 유도된 PC-12 신경세포의 형태학적 관찰
  6. Lactate dehydrogenase (LDH) 측정
  7. Bcl family 단백질 분석
 요약 및 결론
 감사의 글
 References

• 한경훈(서울의료원 의학연구소) | Kyung-Hoon Han (Research Institute, Seoul Medical Center)
• 이승희(서울의료원 의학연구소) | Seung-Hee Lee (Research Institute, Seoul Medical Center)
• 박광성(서울의료원 의학연구소) | Kwang-Sung Park (Research Institute, Seoul Medical Center)
• 송관영(서울의료원 신경외과) | Kwan-Young Song (Dept. of Neurosurgery, Seoul Medical Center)
• 김정희(서울의료원 신경외과) | Jung-Hee Kim (Dept. of Neurosurgery, Seoul Medical Center)
• 박은국(아주대학교 의과대학 의학유전학과) | Eun-Kuk Park (Dept. of Medical Genetics, Ajou University School of Medicine)
• 한성희(고려대학교 보건과학대학 BK21Plus 보건과학사업단) | Sung-Hee Han (BK21Plus, College of Health Science, Korea University) Corresponding author