논문 상세보기
Establishment of bovine tongue epithelium-derived mesenchymal stem cells KCI 등재
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/340434
pp.95-102
Mesenchymal stem cells (MSCs) are multipotent cells able to differentiate into several cell lineages, which has implications for cell therapy and reproductive biotechnologies. Although MSCs have been isolated from many species, including humans and animals, there is limited data on MSCs from large ruminants, such as bovines. In this study, we tried to isolate and characterize bovine tongue tissue-derived MSCs (boT-MSCs) by investigating phenotype morphology, performing proliferation properties, and determining cell surface marker expression patterns, self-renewal, and differentiation potentials. As a result, the boT-MSCs were successfully isolated by collagenase digestion and maintained proliferative capacity until 20 passages. Moreover, the boT-MSCs expressed pluripotency markers (OCT3/4, SOX2, and NANOG) and MSC-specific surface markers including CD44, CD90, and CD105, but not CD45 and MHC-II. The boT-MSCs could also differentiate into mesodermal (adipocyte, osteocyte, and chondrocyte) cell lineages. Our results suggest that the tongues of bovines could be used as a source of MSCs.
재료 및 방법
혀 조직 유래 성체줄기세포 분리
세포 증식률 분석 (Cumulative population doubling level analysis, CPDL)
유세포 분석을 통한 세포표면 특이단백질 발현 검사
Quantitative real-time RT-PCR (qRT-PCR)을 이용한 유전자 발현 검사
중배엽 분화 유도 및 분화능 확인
통계학적 분석
결 과
소 혀 조직 유래 성체줄기세포의 분리 및 증식능 확인
소 혀 조직 유래 성체줄기세포의 세포표면 특이단백질 및 초기전사인자 발현
소 혀 조직 유래 성체줄기세포의 중배엽 분화능 확인
고 찰
감사의 글
REFERENCES
