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Proliferating effects of vegetable resources in equine mesenchymal stem cells KCI 등재
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/341587
pp.113-120
Mesenchymal stem cells (MSCs) have restricted life spans in vitro and can therefore only be expanded for a limited number of cell divisions before entering a senescent state and unequivocally stopping proliferation. Several types of cell culture systems have been used for large-scale expansion of MSCs. A recent trend in cell culture has been the change from serum-use to serum-supplement media. This study was conducted to investigate the proliferative effects of vegetable resources (VR) on equine adipose tissue-derived mesenchymal stem cells (eAD-MSCs) in the absence of serum and their possible mechanisms of action. Regulation of cell cycling is a key process involved in the fate of stem cells, including renewal and differentiation. In this study, we observed that the viability of eAD-MSCs was increased significantly when treated with VR under serum-free conditions. We also observed that expression levels of cell cycling-related proteins such as p53 and p21 were decreased, and proliferating cell nuclear antigen increased significantly in response to treatment with VR in eAD-MSCs under serum-free conditions. Furthermore, expression levels of cell survival-related proteins were increased in response to treatment with VR in eAD-MSCs under serum-free conditions. Therefore, our results suggest that VR promotes proliferation of eAD-MSCs under serum-free conditions.
재료 및 방법
말 지방 조직 유래 중간엽 줄기세포 분리 및 배양
유세포 분석 (flow cytometric analysis)을 통한 세포 표면 특이 단백질 발현 검사
중배엽 분화 유도
식물성 소재의 조제 및 희석
식물성 소재의 세포 처리
세포 생존률 측정
정량적 중합효소연쇄반응 (quantitative real-time RT-PCR)을 이용한 분석
통계학적 분석
결 과
식물성 소재가 중간엽 줄기세포의 세포 생존률에 미치는 영향
식물성 소재가 중간엽 줄기세포의 세포 주기에 미치는 영향
식물성 소재가 중간엽 줄기세포의 세포 노화에 미치는 영향
식물성 소재가 중간엽 줄기세포의 초기전사인자에 미치는 영향
고 찰
감사의 글
REFERENCES
